Near-infrared (NIR) contrast agents are becoming more frequently studied in medical imaging due to their advantageous
characteristics, most notably the ability to capture near-infrared signal across the tissue and the safety of the technique.
This produces a need for imaging technology that can be specific for both the NIR dye and medical application.
Indocyanine green (ICG) is currently the primary NIR dye used in neurosurgery. Here we report on using the augmented
microscope we described previously for image guidance in a rat glioma resection. Luc-C6 cells were implanted in a rat
in the left-frontal lobe and grown for 22 days. Surgical resection was performed by a neurosurgeon using augmented
microscopy guidance with ICG contrast. Videos and images were acquired to evaluate image quality and resection
margins. ICG accumulated in the tumor tissue due to enhanced permeation and retention from the compromised bloodbrain-
barrier. The augmented microscope was capable of guiding the rat glioma resection and intraoperatively
highlighted tumor tissue regions via ICG fluorescence under normal illumination of the surgical field.
Jeffrey R. Watson, Nikolay Martirosyan, Summer Garland, G. Michael Lemole, and Marek Romanowski, "Intraoperative imaging using intravascular contrast agent," Proc. SPIE 9696, Molecular-Guided Surgery: Molecules, Devices, and Applications II, 96960L (Presented at SPIE BiOS: February 14, 2016; Published: 4 March 2016); https://doi.org/10.1117/12.2213867.
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