We used Optical Coherence Microscopy (OCM) to monitor structural and functional changes due to ischemic stroke in
small animals brains in vivo. To obtain lateral resolution of 2.2 μm over the range of 600 μm we used extended focus
configuration of OCM instrument involving Bessel beam. It provided access to detailed 3D information about the
changes in brain vascular system up to the level of capillaries across I and II/III layers of neocortex. We used
photothrombotic stroke model involving photoactive application of rose bengal to assure minimal invasiveness of the
procedure and precise localization of the clot distribution center. We present the comparative analysis involving
structural and angiographic maps of the stroke-affected brain enabling in-depth insight to the process of development of
Szymon Tamborski, Hong Chou Lyu, Danuta Bukowska, Hubert Dolezyczek, Grzegorz Wilczynski, Daniel Szlag, Theo Lasser, Maciej Wojtkowski, and Maciej Szkulmowski, "Imaging of the stroke-related changes in the vascular system of the mouse brain with the use of extended focus optical coherence microscopy," Proc. SPIE 9697, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX, 969718 (Presented at SPIE BiOS: February 16, 2016; Published: 8 March 2016); https://doi.org/10.1117/12.2214779.
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