We present a comprehensive imaging methodology for 3D structural and functional measurements of fertilized mouse oocytes. In contrary to methods used for mouse zygote imaging so far OCT provides 3D data without z axis movement of sample or objective lens. Furthermore, complex scanning protocols used in this study give access to different scales of repetition times and thus may become a tool for investigation of a different dynamic processes. Additionally, proposed scanning approach via variety of statistic operations can be used to enhance the quality of structural images. OCT system capabilities are presented and compared to standard microscopy. With a single 3D measurements one can extract 3D structure of the oocytes as well as en-face images that correspond to both bright and dark field microscopy. As an example of dynamic oocyte imaging pronuclei motion during development is presented. Limitations and possibilities of the new system are discussed.
Karol Karnowski, Anna Ajduk, Maciej Wojtkowski, and Maciej Szkulmowski, "Structural and functional measurements of fertilized mouse oocytes with combined high-resolution OCT and inverted microscope
(Conference Presentation)," Proc. SPIE 9697, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX, 96972B (Presented at SPIE BiOS: February 18, 2016; Published: 26 April 2016); https://doi.org/10.1117/12.2214736.4848635727001.
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