The purpose of this study is to evaluate the effect of a high-lipid diet on elasticity of adipose tissue. We employed dual Raman/Brillouin microspectroscopy to analyze brown and white adipose tissues obtained from adult rats. The rats were divided into two groups, one of which received a high-fat feed, while the other served as a control. We hypothesized that the changes in the elasticity of adipose tissues between the two groups can be successfully assessed using Brillouin spectroscopy. We found that the brown adipose tissue possessed a lesser Brillouin shift than the white adipose within each group and that the elastic modulus of both adipose tissues increases in the high-fat diet group. The Raman spectra provided supplementary chemical information and indicated an increase in the lipid-to-protein ratio in the brown adipose, but not in the white adipose.
Maria Troyanova-Wood, Cassidy Gobbell, Zhaokai Meng, and Vladislav V. Yakovlev, "Assessing the effect of a high-fat diet on rodents' adipose tissue using Brillouin and Raman spectroscopy," Proc. SPIE 9703, Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis, 970310 (Presented at SPIE BiOS: February 17, 2016; Published: 7 March 2016); https://doi.org/10.1117/12.2213525.
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