Urine is considered diagnostically important for tits native fluorophores and they vary in their distribution, concentration and physiochemical properties, depending upon the metabolic condition of the subject. In this study, we have made an attempt, to characterize the urine of normal subject and diabetic patients under medication by native fluorescence spectroscopy at 280 nm excitation. Further, the fluorescence data were analyzed employing the multivariate statistical method linear discriminant analysis (LDA) using leave one out cross validation method. The results were promising in discriminating diabetic urine from that of normal urine. This study in future may be extended to check the feasibility in ruling out the coexisting disorders such as cancer.
Anjana Kesavan, Rekha Pachaiappan, Prakasa Rao Aruna, and Singaravelu Ganesan, "Steady state fluorescence spectroscopic characterization of normal and diabetic urine at selective excitation wavelength 280 nm," Proc. SPIE 9703, Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis, 97031H (Presented at SPIE BiOS: February 18, 2016; Published: 7 March 2016); https://doi.org/10.1117/12.2212625.
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