Signal transductions including multiple protein post-translational modifications (PTM), protein-protein interactions (PPI), and protein-nucleic acid interaction (PNI) play critical roles for cell proliferation and differentiation that are directly related to the cancer biology. Traditional methods, like mass spectrometry, immunoprecipitation, fluorescence resonance energy transfer, and fluorescence correlation spectroscopy require a large amount of sample and long processing time. “microchannel for multiple-parameter analysis of proteins in single-complex (mMAPS)”we proposed can reduce the process time and sample volume because this system is composed by microfluidic channels, fluorescence microscopy, and computerized data analysis. In this paper, we will present an automated mMAPS including integrated microfluidic device, automated stage and electrical relay for high-throughput clinical screening. Based on this result, we estimated that this automated detection system will be able to screen approximately 150 patient samples in a 24-hour period, providing a practical application to analyze tissue samples in a clinical setting.
Po-Jung Huang, Sina Baghbani Kordmahale, Chao-Kai Chou, Hirohito Yamaguchi, Mien-Chie Hung, and Jun Kameoka, "Development of automated high throughput single molecular microfluidic detection platform for signal transduction analysis," Proc. SPIE 9705, Microfluidics, BioMEMS, and Medical Microsystems XIV, 970511 (Presented at SPIE BiOS: February 15, 2016; Published: 21 March 2016); https://doi.org/10.1117/12.2213259.
Conference Presentations are recordings of oral presentations given at SPIE conferences and published as part of the proceedings. They include the speaker's narration with video of the slides and animations. Most include full-text papers. Interactive, searchable transcripts and closed captioning are now available for 2018 presentations, with transcripts for prior recordings added daily.
Search our growing collection of more than 16,000 conference presentations, including many plenaries and keynotes.