We present an optical coherence tomography (OCT) based method for mapping transverse red blood cell (RBC) flow speed at capillary. This OCT velocimetry utilizes a quantitative laser speckle temporal contrast analysis that estimates reliable speckle decorrelation time from the observed speckle contrast, which is related to microcirculatory flow velocity. For capillary speed measurement, we employ a home-built 1.3 µm MHz swept-source OCT (SS-OCT) system that can acquire OCT B-frames at a rate of 1.7 kHz. From the multiple B-frames obtained at the same location, intensity profiles with time-varying OCT speckle contrast are extracted at single capillaries using a capillary binary mask and then the transverse flow speed is calculated by adapting the profiles to the speckle contrast analytic model. Finally, a 3D speed map can be achieved for OCT volume imaging. To validate this method, we perform a systematic study using both phantom and in vivo rodent models. Result shows that our method is effective to measure transverse capillary flow speed.
Woo June Choi and Ruikang K. Wang, "Mapping transverse capillary flow speed using time-varying OCT speckle signals
(Conference Presentation)," Proc. SPIE 9707, Dynamics and Fluctuations in Biomedical Photonics XIII, 970707 (Presented at SPIE BiOS: February 14, 2016; Published: 26 April 2016); https://doi.org/10.1117/12.2214001.4848677980001.
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