In this study, we demonstrate that OCT speckle decorrelation techniques can be used to probe intracellular motion in cancer cells. Spheroids and cell pellets were used as a model to probe intracellular motion. ZnCl2 was used to inhibit mitochondrial motion within the cells. The results reveal the changes in intracellular motion during the spheroid growth phase. Moreover, to modify the motion of mitochondria, cell pellet were exposed to ZnCl2, and agent known to o impair cellular energy production through inhibition of mitochondrial function. The speckle decorrelation time during the growth phase of spheroids decreased by 35 ms over 21 days and 25 ms during inhibition of mitochondrial motion 10 minutes after exposure to ZnCl2.
Azhar Zam and Michael C. Kolios, "Measuring intracellular motion in cancer cell using optical coherence tomography," Proc. SPIE 9707, Dynamics and Fluctuations in Biomedical Photonics XIII, 97070V (Presented at SPIE BiOS: February 15, 2016; Published: 17 March 2016); https://doi.org/10.1117/12.2209652.
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