Cytotoxic T lymphocytes (CTLs) played a key role in the immune system to destroy the tumor cells. Although some mechanisms of CTLs killing the tumor cells are revealed already, the dynamic information of CTLs interaction with tumor cells are still not known very clearly. Here we used confocal microscopy to visualize the whole process of CTLs killing the tumor cells in vitro. The imaging data showed that CTLs destroyed the target tumor cells rapidly and efficiently. Several CTLs surrounded one or some tumor cells and the average time for CTLs destroying one tumor cell is just a few minutes in vitro. The study displayed the temporal events of CTLs interacting with tumor cells at the beginning and finally killing them and directly presented the efficient tumor cell cytotoxicity of the CTLs. The results helped us to deeply understand the mechanism of the CTLs destroying the tumor cells and to develop the cancer immunotherapy.
Shuhong Qi and Zhihong Zhang, "Dynamic visualization the whole process of cytotoxic T lymphocytes killing the B16 tumor cells in vitro," Proc. SPIE 9709, Biophotonics and Immune Responses XI, 970908 (Presented at SPIE BiOS: February 15, 2016; Published: 15 March 2016); https://doi.org/10.1117/12.2214268.
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