Coherent Raman imaging methods have been under development for almost 15 years. The field is beginning to mature, transitioning from a “new techniques” phase to an applications phase. I will discuss current capabilities of broadband coherent anti-Stokes Raman scattering (BCARS) microscopy using optimized excitation paradigms, and provide a few examples of how broadband BCARS imaging has helped to answer (or raise) questions in investigations of tissues and small organisms. I will also discuss progress in processing BCARS spectra to make them independent of excitation profile or non-resonant response, and directly comparable to spontaneous Raman spectra. I will also discuss progress on a new approach to time-domain BCARS that promises to significantly simplify and speed BCARS data acquisition.
Marcus T. Cicerone and Charles H. Camp, "Rapid label-free chemical imaging of cells and tissues
(Conference Presentation)," Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 97120C (Presented at SPIE BiOS: February 14, 2016; Published: 27 April 2016); https://doi.org/10.1117/12.2216118.4848767261001.
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