We have developed a coherent Raman scattering microscope that combines total internal reflection illumination with surface plasmon resonance. The excitation geometry is based on an objective-type Kretschmann configuration, which allows widefield excitation of surface plasmon polariton modes in a thin gold film on a glass substrate. The surface plasmon fields enhance the excitation efficiency, enabling image acquisition at 10 frames/s. Since the evanescent field extends only over a length scale of ~100 nm, structures close the substrate surface are observed while bulk contributions are suppressed. We discuss the operational principles of this microscope in detail and point out its applications in cell biology.
Eric O. Potma, Alex Fast, and Christropher D. Syme, "Surface-enhanced coherent Raman scattering
(Conference Presentation)," Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 97120I (Presented at SPIE BiOS: February 14, 2016; Published: 27 April 2016); https://doi.org/10.1117/12.2213662.4848767231001.
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