Second harmonic generation (SHG) is a powerful technique to observe fibrillar collagen without any staining and with a good contrast. More information about the molecular structure of collagen fibrils in tissues and their 3D distribution can be gained with polarization-resolved SHG imaging. Nevertheless, strong focusing is required for effective imaging and light propagation in tissues is complex and not thoroughly understood yet, preventing accurate and reproducible measurements. Theoretical analysis, vectorial numerical simulations and experiments were implemented to understand how the SHG signal builds up and how geometrical parameters affect polarization-resolved measurements in homogeneous collagen-rich tissues.
Claire Teulon, Ivan Gusachenko, Gaël Latour, and Marie-Claire Schanne-Klein, "Forward versus backward polarization-resolved SHG imaging of collagen structure in tissues," Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 971218 (Presented at SPIE BiOS: February 16, 2016; Published: 14 March 2016); https://doi.org/10.1117/12.2208110.
Conference Presentations are recordings of oral presentations given at SPIE conferences and published as part of the proceedings. They include the speaker's narration with video of the slides and animations. Most include full-text papers. Interactive, searchable transcripts and closed captioning are now available for most presentations.
Search our growing collection of more than 22,000 conference presentations, including many plenaries and keynotes.