Femtosecond near infrared laser microscopes are widely used to perform high resolution 3D imaging of biological samples based on second harmonic generation (SHG) and non-resonant simultaneous absorption of two or more photons at GW/cm2 intensities. However, high contrast imaging of living specimens without any destructive effect is limited to certain laser and exposure parameters with respect to the optical properties of the target.
We compared three different femtosecond lasers, including a novel ultra-compact ultrashort fiber laser, in the range of 15-180 fs and repetition rates of 50–300 MHz for optimal non-destructive two-photon autofluorescence imaging. In particular we determined the thresholds for the onset of photodamage effects such as impaired cell reproduction.
Aisada Uchugonova, Hans Georg Breunig, Tuan Li, and Karsten König, "Multiphoton tomography with tunable Ti:sapphire laser
(Conference Presentation)," Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 97121B (Presented at SPIE BiOS: February 16, 2016; Published: 27 April 2016); https://doi.org/10.1117/12.2217580.4848767248001.
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