We present a novel super-resolution spectral encoding of spatial frequency (srSESF) approach to break the diffraction limit and dramatically improve resolution in lateral direction. The idea is to utilize additional information about the internal structure of the object to resolve features in the lateral direction. The novel contrast mechanism is realized by reconstruction and comparison of the axial spatial frequency (period) profiles at each image point to form super-resolution image. As a result, small features, unresolved by conventional microscopy, can be visualized. Numerical simulation and experiments confirm the super-resolution abilities of the srSESF approach without applying any labels.
Sergey A. Alexandrov, James McGrath, Hrebesh Subhash, Francesca Boccafoschi, Cinzia Giannini, and Martin Leahy, "Novel contrast mechanism for label free super-resolution imaging," Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 971303 (Presented at SPIE BiOS: February 15, 2016; Published: 9 March 2016); https://doi.org/10.1117/12.2207458.
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