Three-dimensional real-time imaging is a fundamental challenge for understanding dynamic processes. In this presentation, we propose a new method based on a tunable acoustic gradient lens integrated in a simple optical system. By synchronizing a pulsed LED with a high-speed camera, we are able to resolve a volume of 2 millimeters and 2 millimeters with depth 1 millimeter in 7 microseconds. A simulation model of the optical system is provided and serves as a useful tool for designing the optical system for the desired aspect-ratio of the imaging volume. The ability to resolve a volume in microseconds opens the door to exploring the fundamental dynamics in micro scale.
Ting Hsuan Chen and Craig B. Arnold, "Volumetric real-time wide-field microscopy with tunable acoustic gradient lens
(Conference Presentation)," Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 971307 (Presented at SPIE BiOS: February 15, 2016; Published: 27 April 2016); https://doi.org/10.1117/12.2212604.4848767600001.
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