I present multimodal wide-field interferometric microscopy platform for label-free 3-D imaging of live cells during fast flow. Using holographic optical tweezers, multiple cells can be optically trapped and rapidity rotated on all axes, while acquired using an external off-axis wide-field interferometric module developed in our lab. The interferometric projections are rapidly processed into the 3-D refractive-index profile of the cells using a tomographic phase microscopy algorithms that take into consideration optical diffraction effects. The algorithms for the 3-D refractive-index reconstruction, and for calculating various morphological parameters that should serve for online sorting of cells, are efficiently implemented in a nearly real-time manner. The potential of this new high-throughput imaging technique is for label-free image analysis and sorting of cells during flow, to substitute current cell sorting devices, which are based on external labeling that eventually damages the cell sample.
Natan Tzvi Shaked, "Multimodal interferometric microscopy for label-free 3D imaging of live cells in flow
(Conference Presentation)," Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 97130C (Presented at SPIE BiOS: February 15, 2016; Published: 27 April 2016); https://doi.org/10.1117/12.2214447.4848767264001.
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