This work presents a technique for a full 3D imaging of biological samples tagged with gold-nanoparticles (GNPs) using only two images, rather than many images per volume as is currently needed for 3D optical sectioning microscopy. The proposed approach is based on the Gerchberg-Saxton (GS) phase retrieval algorithm. The reconstructed field is free space propagated to all other focus planes using post processing, and the 2D z-stack is merged to create a 3D image of the sample with high fidelity. Because we propose to apply the phase retrieving on nano particles, the regular ambiguities typical to the Gerchberg-Saxton algorithm, are eliminated. In addition, since the method requires the capturing of two images only, it can be suitable for 3D live cell imaging. The proposed concept is presented and validated both on simulated data as well as experimentally.
Tali Ilovitsh, Asaf Ilovitsh, Aryeh Weiss, Rinat Meir, and Zeev Zalevsky, "Three-dimensional imaging using phase retrieval with two focus planes," Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 97130Y (Presented at SPIE BiOS: February 16, 2016; Published: 9 March 2016); https://doi.org/10.1117/12.2205769.
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