We present a deformable mirror based remote focusing method for three-dimensional imaging in high-resolution microscopy systems. The method relies on predefined mirror mode arrays that are obtained during initial mirror training step with a low complexity wavefront-sensing module. The imaging plane can be refocused over distances over a hundred times greater than the original depth of field of the objective lens along the optical axis at millisecond rates. We will demonstrate the combination of the remote focusing method with spatiotemporally focused two-photon excitation applied to three-dimensional imaging of biological samples.
Mantas Zurauskas, Maria Frade, and Martin Booth, "Deformable mirror based remote focusing for fast three-dimensional microscopy," Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 971313 (Presented at SPIE BiOS: February 16, 2016; Published: 9 March 2016); https://doi.org/10.1117/12.2212335.
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