This overview talk will focus on forward-looking scientific needs and physical limits to images of neuronal processes. The challenge in nervous systems is that the basic unit for "switching" events in the nervous system occurs on the one micrometer scale of synaptic spines, while computations involve communication between individual neurons across the full expanse of cortex, which is ten millimeters for mouse cortex. I will address hoped-for advances in optical microscopy, within the context of existing and proposed contrast mechanisms of neuronal function, that span the four orders of magnitude of length scales for neuronal processing
David Kleinfeld, "What advances in microscopy are required for combined MRI and optical functional brain imaging?
(Conference Presentation)," Proc. SPIE 9717, Adaptive Optics and Wavefront Control for Biological Systems II, 97170D (Presented at SPIE BiOS: February 13, 2016; Published: 27 April 2016); https://doi.org/10.1117/12.2218121.4848767664001.
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