Second-harmonic generation (SHG) has proven to be an effective method to both image and detect structural
variations in fibrillar collagen. The ability to detect these differences is especially useful in studying diseases
like cancer and fibrosis.1 SHG techniques have historically been limited by their ability to penetrate and image
through strongly scattering tissues. Recently, optical wavefront shaping has enabled light to be focused through
highly scattering media such as biological tissue.2-4 This technology also enables us to examine the dependence
of second harmonic generation on the spatial phase of the pump laser. Here, we demonstrate that wavefront
shaping can be used to enhance the generation of second harmonic light from collagen fibrils even when scattering
is low or non-existent.
Jonathan V. Thompson, Graham A. Throckmorton, Brett H. Hokr, and Vladislav V. Yakovlev, "Enhanced second-harmonic-generation detection of collagen by means of optical wavefront shaping," Proc. SPIE 9717, Adaptive Optics and Wavefront Control for Biological Systems II, 97170N (Presented at SPIE BiOS: February 14, 2016; Published: 15 March 2016); https://doi.org/10.1117/12.2213644.
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