Of the large amount of the animal models available for cardiac research, the zebrafish is extremely valuable due to its transparency during early stages of development. In this work a dual illumination laser sheet microscope with simultaneous dual camera imaging is used to image the beating heart at 200 fps, dynamically and selectively focusing inside the beating heart through the use of a tunable lens. This dual color dynamic focusing enables imaging with cellular resolution at unprecedented high frame rates, allowing 3D imaging of the whole beating heart of embryonic zebrafish.
L. Andrés-Delgado, M. Peralta, N. Mercader, and J. Ripoll, "Dynamic focusing in the zebrafish beating heart," Proc. SPIE 9717, Adaptive Optics and Wavefront Control for Biological Systems II, 971717 (Presented at SPIE BiOS: February 15, 2016; Published: 15 March 2016); https://doi.org/10.1117/12.2211739.
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