Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is standard selective plane illumination microscope (SPIM) configuration modified with the addition of a spatial light modulator (SLM) and a third objective for the detection of transmitted light. Optimization protocols use this transmission light allowing the extension the depth-of-field and correction of aberrations whilst retaining a thin optical section.
Dean Wilding, Paolo Pozzi, Oleg Soloviev, Gleb Vdovin, and Michel Verhaegen, "Light-sheet optimization for microscopy," Proc. SPIE 9717, Adaptive Optics and Wavefront Control for Biological Systems II, 971718 (Presented at SPIE BiOS: February 15, 2016; Published: 15 March 2016); https://doi.org/10.1117/12.2210912.
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