Biophotonic studies based on partial wave spectroscopy have shown that early carcinogenesis is characterized by a change in the nanoscale molecular organization of the cell nuclii. These finding suggest that cancer is associated with change in macromolecular crowding. In this presentation we will discuss a recent approach that we have developed to incorporate molecular scale information into a systems based approach to study the role of macromolecular crowding on different phenomena ranging from protein diffusion to gene transcription. Macromolecular crowding affects both dynamics and equilibrium properties. We will show that transcription is a non-monotonic function of crowders concentration in the cell nuclei. Furthermore, we will show how changes in macromolecular crowding in the nuclei and in the cytoplasm lead to different changes in the oscillatory behavior on NF-κB upon stimuli. Our results show the important regulatory role that non-specific interactions play in biological systems.
Igal Szleifer, "Crowding, dynamics and transcription
(Conference Presentation)," Proc. SPIE 9719, Biophysics, Biology, and Biophotonics: the Crossroads, 971908 (Presented at SPIE BiOS: February 13, 2016; Published: 27 April 2016); https://doi.org/10.1117/12.2213461.4848767881001.
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