We propose a novel fluorescence microscopy technique to study the adhesion of Giant Unilamellar Vesicles. Their adhesion is assessed through the normalization of Total Internal Reflection Fluorescence (TIRF) images by epi-fluorescence ones. This can be achieved by using a motorized rotatable mirror to switch from epi-fluorescence to TIRF. This method allows us to study the vesicle adhesion from ultra-weak to strong membrane surface interactions and to measure the absolute distance between the vesicles and various chemically functionalized glass substrates at the nanoscale.
Marcelina Cardoso Dos Santos, Cyrille Vézy, and Rodolphe Jaffiol, "Vesicle adhesion depicted at nanoscale with normalized total internal reflection fluorescence microscopy," Proc. SPIE 9719, Biophysics, Biology, and Biophotonics: the Crossroads, 97190I (Presented at SPIE BiOS: February 14, 2016; Published: 9 March 2016); https://doi.org/10.1117/12.2208429.
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