There is an increasing interest in optical reporters like semiconductor quantum dots and upconversion nanophosphors with emission < 1000 nm for bioanalysis, medical diagnostics, and safety barcodes and hence, in reliable fluorescence measurements in this wavelength region, e.g., for the comparison of material performance and the rational design of new nanomaterials with improved properties [1-4].
The performance of fluorescence measurements < 800 nm and especially < 1000 nm is currently hampered by the lack of suitable methods and standards for the simple determination of the wavelength-dependent spectral responsivity of fluorescence measuring systems and the control of measured emission spectra and intensities [3-5]. This is of special relevance for nanocrystalline emitters like quantum dots and rods as well as for upconversion nanocrystals, where surface states and the accessibility of emissive states by quenchers largely control accomplishable quantum yields and hence, signal sizes and detection sensitivities from the reporter side.
Here, we present the design of an integrating sphere setup for the absolute measurement of emission spectra and quantum yields in the wavelength region of 650 to 1600 nm and its calibration as well as examples for potential fluorescence standards from different reporter classes for the control of the reliability of such measurements . This includes new spectral fluorescence standards for the wavelength region of 650 nm to 1000 nm as well as a set of quantum yield standards covering the wavelength region from 400 nm to 1000 nm.
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