The interaction of light with biological cells can provide a unique tool for studying their biophysical properties. Optical spectroscopy of biological cells can reveal detailed information on their structure and dynamics in a way that is not possible with traditional microscopy techniques. Histological evaluation can only obtain a snapshot of the activity of individual cells, relying instead on large ensembles to develop a picture of their temporal evolution. On the other hand optical spectroscopy can be applied to cells with little to no preparation and can enable studies of the same live cells at extended time intervals. Our research group has developed a suite of optical spectroscopic tools to assess the structure and function of biological cells and modulation due to the onset of disease. The wavelength dependence of the interaction of cells with light provides information of cell features through elastic scattering across the visible and near infrared spectrum. Alternatively, the angular dependence of scattered light can also be used to reveal cell properties. We will discuss how both modes of elastic scattering can be used to evaluate cell status. Finally, the recent advances in the use of optical phase imaging to create contrast in nearly transparent biological cells will also be discussed as related to the role of this modality in biosensing.
Adam Wax, "Cellular biosensing using optical spectroscopy
(Conference Presentation)," Proc. SPIE 9725, Frontiers in Biological Detection: From Nanosensors to Systems VIII, 972502 (Presented at SPIE BiOS: February 14, 2016; Published: 27 April 2016); https://doi.org/10.1117/12.2217439.4848770191001.
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