We report an optical cavity based biosensor using a novel differential detection method for point-of-care applications. Two laser diodes allow for multiplexing capability along with the ability to enhance the responsivity using differential detection. The laser wavelengths are chosen so that the optical intensities of two lasers change monotonically with opposite slopes upon the adsorption of desired biomarkers. The cavity width, PMMA thickness, and silver thickness have been optimized to achieve a large change in scaled differential value. We chose biotinylated BSA detection with Avidin as a receptor molecule to demonstrate the proposed design. Avidin is attached directly to the PMMA layer by physisorption. Then, biotinylated BSA is introduced to the sample and the intensities of the laser diodes are measured by a sCMOS camera. A change in the scaled differential value will correlate to the binding of biotinylated BSA. In this presentation, we will discuss simulation results, fabrication procedures, and preliminary measurement results.
Peter Cowles, Cody Joy, Antonio Bujana, DongGee Rho, and Seunghyun Kim, "Preliminary measurement results of biotinylated BSA detection of a low cost optical cavity based biosensor using differential detection," Proc. SPIE 9725, Frontiers in Biological Detection: From Nanosensors to Systems VIII, 972509 (Presented at SPIE BiOS: February 15, 2016; Published: 22 April 2016); https://doi.org/10.1117/12.2212904.
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