G-quadruplex, readily formed by the G-rich sequence, potentially distributes in over 40 % of all human genes, such as the telomeric DNA with the G-rich sequence found at the end of the chromosome. The G-quadruplex structure is supposed to possess a diverse set of critical functions in the mammalian genome for transcriptional regulation, DNA replication and genome stability. However, most of the currently available methods for G-quadruplex identification are restricted to fluorescence techniques susceptible to poor sensitivity. It is essential to propose methods with higher sensitivity to specifically recognize the G-quadruplexes. In this study, we demonstrate a label-free plasmonic biosensor for G-quadruplex detection by relying on the advantages of nanoporous gold (NPG) disks that provide high-density plasmonic hot spots, suitable for molecular recognition capability without the requirement for labeling processes.
Suyan Qiu, Fusheng Zhao, Greggy M. Santos, and Wei-Chuan Shih, "Plasmonic biosensor for label-free G-quadruplexes detection," Proc. SPIE 9725, Frontiers in Biological Detection: From Nanosensors to Systems VIII, 97250F (Presented at SPIE BiOS: February 15, 2016; Published: 22 April 2016); https://doi.org/10.1117/12.2212318.
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