We present experimental studies of live cancer cells via microscopic imaging ellipsometry (MIE). The Rotating Compensator Ellipsometry (RCE) is used for our measurements. Ellipsometry spectra with signals integrated over a 20μm×20μm area in visible range (450nm to 750nm) are obtained with the Optrel MULTISKOP system for both specular reflection and off-specular scattering. The microscopic ellipsometry (ME) images at a few fixed wavelengths within the same range were also analyzed. Dielectric constants for glass slide, culture fluid, and glass cover plate were firstly characterized by spectroscopic ellipsometry measurements, which can be used in the simulation for MIE measurements of cancer cells embedded in the culture fluid and sandwiched between a glass slide and cover plate. The measured ME spectra and images of cancer cells before and after medicine injection are measured and analyzed.
Yu-Da Chen, Hao Yun Hsu, Mai Ibrahim Khaleel, Yia-Chung Chang, Chien-Hsun Wu, and Han-Chung Wu, "Study of biological reaction in cancer cell with spectroscopic imaging ellipsometry," Proc. SPIE 9925, Nanoimaging and Nanospectroscopy IV, 992505 (Presented at SPIE Nanoscience + Engineering: August 28, 2016; Published: 15 September 2016); https://doi.org/10.1117/12.2236288.
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Study of self-shadowing effect as a simple means to realize nanostructured thin films and layers with special attentions to birefringent obliquely deposited thin films and photo-luminescent porous silicon