Recent experimental Dynamic Light Scattering (DLS) studies of the coil sizes of DNA-CTMA:Rh solutions have lead to numerical discrepancies with theoretical predictions amounting to one-two orders of magnitude.1 In this paper, which has partially character of a tutorial, we present the basic theoretical concepts underlying an analysis of the polymer coil sizes from DLS experiments. In particular, we discuss the limitations of those methods. We present a wormlike model of a polymer chain which is a promising candidate for inferring information about the spatial structure of the DNA chain from experimental data.
A. C. Mitus, W. Radosz, G. Pawlik, C. A. Lazar, F. Kajzar, and I. Rau, "Dynamical light scattering for DNA-CTMA:DR1 chains: wormlike semi-flexible model, coil size and persistence length," Proc. SPIE 9928, Nanobiosystems: Processing, Characterization, and Applications IX, 992807 (Presented at SPIE Nanoscience + Engineering: August 28, 2016; Published: 13 September 2016); https://doi.org/10.1117/12.2238000.
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Study of self-shadowing effect as a simple means to realize nanostructured thin films and layers with special attentions to birefringent obliquely deposited thin films and photo-luminescent porous silicon