From Event: SPIE Optical Engineering + Applications, 2016
X-ray imaging in absorption contrast mode is well established for hard tissue visualization. However, performance
for lower density materials is limited due to a reduced contrast. Our aim is three-dimensional (3D)
characterization of micro-morphology of human brain tissues down to (sub-)cellular resolution within a laboratory
environment. Using the laboratory-based microtomography (μCT) system nanotom m (GE Sensing
and Inspection Technologies GmbH, Wunstorf, Germany) and synchrotron radiation at the Diamond-Manchester
Imaging Branchline I13-2 (Diamond Light Source, Didcot, UK), we have acquired 3D data with a resolution
down to 0.45 μm for visualization of a human cerebellum specimen down to cellular level. We have shown that
all selected modalities, namely laboratory-based absorption contrast micro-tomography (LBμCT), synchrotron
radiation based in-line single distance phase contrast tomography (SDPR) and synchrotron radiation based
single-grating interferometry (GI), can reach cellular resolution for tissue samples with a size in the mm-range.
The results are discussed qualitatively in comparison to optical microscopy of haematoxylin and eosin (HE)
stained sections. As phase contrast yields to a better data quality for soft tissues and in order to overcome
restrictions of limited beamline access for phase contrast measurements, we have equipped the μCT system
nanotom m with a double-grating phase contrast set-up. Preliminary experimental results of a knee sample
consisting of a bony part and a cartilage demonstrate that phase contrast data exhibits better quality compared
to absorption contrast. Currently, the set-up is under adjustment. It is expected that cellular resolution would
also be achieved. The questions arise (1) what would be the quality gain of laboratory-based phase contrast in
comparison to laboratory-based absorption contrast tomography and (2) could laboratory-based phase contrast
data provide comparable results to synchrotron radiation based phase contrast data.
Anna Khimchenko, Georg Schulz, Hans Deyhle, Peter Thalmann, Irene Zanette, Marie-Christine Zdora, Christos Bikis, Alexander Hipp, Simone E. Hieber, Gabriel Schweighauser, Jürgen Hench, and Bert Müller, "X-ray micro-tomography for investigations of brain tissues on cellular level," Proc. SPIE 9967, Developments in X-Ray Tomography X, 996703 (Presented at SPIE Optical Engineering + Applications: August 29, 2016; Published: 4 October 2016); https://doi.org/10.1117/12.2237554.
Conference Presentations are recordings of oral presentations given at SPIE conferences and published as part of the proceedings. They include the speaker's narration with video of the slides and animations. Most include full-text papers. Interactive, searchable transcripts and closed captioning are now available for most presentations.
Search our growing collection of more than 22,000 conference presentations, including many plenaries and keynotes.