From Event: SPIE BiOS, 2022
Full Field Optical Transmission Tomography (FFOTT) relies on forward scattering using the Gouy’s phase shift modulation that is achieved close to the focus of a microscope objective. FFOTT principle is different than Full Field OCT (FFOCT) that relies on backscattering of light, This new type of endogenous cell imaging technique offers structural and metabolic contrasts and is particularly well suited for imaging cell culture on glass slide or Petri dishes avoiding fringes that mask cells in FFOCT as well as biological structures such as biofilms. The sectioning ability is close to confocal microscopy but no contrast agent is required.
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Albert Claude Boccara, Olivier Thouvenin, Linh Giang Pham, and Martine Boccara, "Endogenous transmission optical tomography of biological samples: static and dynamic approaches," Proc. SPIE PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, PC119720L (Presented at SPIE BiOS: January 24, 2022; Published: 2 March 2022); https://doi.org/10.1117/12.2607849.6295505607001.