At the early onset of a disease, the morphological changes manifest themselves on a cellular level (submicron scale). Label-free detection of nano-scale structural changes in sub-micron structure poses a significant challenge to researchers. A nano-sensitive OCT (nsOCT) technique permits to visualize 3D structure with nano-sensitivity to structural changes. Here, we present a brief theory and further development of the nsOCT approach, demonstrate different imaging modalities and unique abilities by monitoring internal nano-scale structural changes within different objects, including human skin in vivo. The presented results show new possibilities for study of structural changes, without the need for biomarkers or labels.

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Sergey A. Alexandrov, Anand Arangath, Rajib Day, Ryan McAuley, and Martin Leahy, "Visualization of the sub-micron structure of scattering objects with nano-sensitivity," Proc. SPIE PC12378, Dynamics and Fluctuations in Biomedical Photonics XX, PC123780C (Presented at SPIE BiOS: January 30, 2023; Published: 7 March 2023); https://doi.org/10.1117/12.2658015.6322015967112.