An improved fluorescence collection method for two-photon imaging endoscopy

Sertac G. Yazgi; Berk Camli; Liam P. Andrus; Adela Ben-Yakar

doi:10.1117/12.2610023

7 March 2022 An improved fluorescence collection method for two-photon imaging endoscopy

Sertac G. Yazgi, Berk Camli, Liam P. Andrus, Adela Ben-Yakar

Proceedings Volume PC11937, Endoscopic Microscopy XVII; PC119370M (2022) https://doi.org/10.1117/12.2610023
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

A scanning fiber endoscope system utilizing an alternative collection method for improved collection efficiency is presented. Low probability of two-photon excitation and high tissue scattering make signal collection a challenging aspect of two-photon imaging. Our work demonstrates a collection strategy using a high NA optical fiber bundle utilized in addition to the excitation fiber. This scheme significantly increases collection area without requiring collection optics at distal end. Experimentally determined collection efficiency of the method will be compared against our table-top two-photon microscope setup. Various effects of increased collection area on efficiency will be discussed with simulation results.

Conference Presentation

Citation Download Citation

Sertac G. Yazgi, Berk Camli, Liam P. Andrus, and Adela Ben-Yakar "An improved fluorescence collection method for two-photon imaging endoscopy", Proc. SPIE PC11937, Endoscopic Microscopy XVII, PC119370M (7 March 2022); https://doi.org/10.1117/12.2610023

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