Moxifloxacin-based extended depth-of-field fluorescence microscopy for real-time conjunctival goblet cell examination

Jungbin Lee; Seonghan Kim; Byeong Jae Son; Jeongho Kim; Myoung Joon Kim; Chang Ho Yoon; Hong Kyun Kim; Ki Hean Kim

doi:10.1117/12.2610725

7 March 2022 Moxifloxacin-based extended depth-of-field fluorescence microscopy for real-time conjunctival goblet cell examination

Jungbin Lee, Seonghan Kim, Byeong Jae Son, Jeongho Kim, Myoung Joon Kim, Chang Ho Yoon, Hong Kyun Kim, Ki Hean Kim

Author Affiliations +

Proceedings Volume PC11941, Ophthalmic Technologies XXXII; PC119410Y (2022) https://doi.org/10.1117/12.2610725
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Mucin secretive conjunctival goblet cells (CGCs) in the eye are important for tear film stability and ocular surface health. Because CGC dysfunction is associated with various ocular surface diseases, non-invasive CGC examination will be of great help in the diagnosis and treatment. In this study, we developed a high-speed moxifloxacin-based extended depth-of-field microscopy for real-time CGC examination. The performance was demonstrated by high-speed CGC imaging of both mouse and rabbit models, in vivo. The imaging was insensitive to breathing motion, and the image resolution was sufficient to resolve individual CGCs in rabbit models.

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Jungbin Lee, Seonghan Kim, Byeong Jae Son, Jeongho Kim, Myoung Joon Kim, Chang Ho Yoon, Hong Kyun Kim, and Ki Hean Kim "Moxifloxacin-based extended depth-of-field fluorescence microscopy for real-time conjunctival goblet cell examination", Proc. SPIE PC11941, Ophthalmic Technologies XXXII, PC119410Y (7 March 2022); https://doi.org/10.1117/12.2610725

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