Multiphoton microscopy, invented by Denk, Strickler and Webb in 1990, has changed how we visualize cells by providing high-resolution, non-invasive imaging capability deep within intact biological tissues. In vivo, cellular resolution fluorescence imaging is now routinely achievable at greater than 1 mm depth in mouse brains. In this talk, I will present research on pushing the depth limit of long wavelength 3-photon microscopy. I will reflect on my journey in multiphoton imaging and the enormous impact Watt Webb has had on me in science and in life.
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