Fluorescence-guided optical photothermal infrared (O-PTIR) spectroscopy

Craig B. Prater; Mustafa Kansiz; Kevin Kjoller; Roshan Shetty; Ferenc Borondics; Oxana Klementieva

doi:10.1117/12.2615057

2 March 2022 Fluorescence-guided optical photothermal infrared (O-PTIR) spectroscopy

Craig B. Prater, Mustafa Kansiz, Kevin Kjoller, Roshan Shetty, Ferenc Borondics, Oxana Klementieva

Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC119730Q (2022) https://doi.org/10.1117/12.2615057
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We have developed a new multimodal microscope enabling “Fluorescence-Guided” Optical Photothermal Infrared (O-PTIR) spectroscopy. O-PTIR has proven itself a breakthrough in IR spectroscopy, providing IR spectroscopic analysis with spatial resolution ~20x better than traditional IR in a non-contact optical configuration. We have now coupled O-PTIR with fluorescence microscopy, a long established, cornerstone technique in life science research. The new fluorescence guided O-PTIR instrument allows: (1) a sample to be analyzed via fluorescence microscopy to map sample regions that are labeled one or more fluorophores; and (2) use fluorescence images to direct infrared spectroscopic analysis in the vicinity of the fluorescently labeled regions. We have used fluorescence guided O-PTIR to perform spectroscopic analysis of the protein secondary structure of fluorescently labeled amyloid plaques in mouse brain tissue, with the goal of providing insights in neurodegenerative disease research.

Conference Presentation

Citation Download Citation

Craig B. Prater, Mustafa Kansiz, Kevin Kjoller, Roshan Shetty, Ferenc Borondics, and Oxana Klementieva "Fluorescence-guided optical photothermal infrared (O-PTIR) spectroscopy", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730Q (2 March 2022); https://doi.org/10.1117/12.2615057

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