Open access single objective light-sheet microscopy (Conference Presentation)

Manish Kumar; Jose Ernesto Canton-Josh; Yevgenia Kozorovitskiy

doi:10.1117/12.2654989

16 March 2023 Open access single objective light-sheet microscopy (Conference Presentation)

Manish Kumar, Jose Ernesto Canton-Josh, Yevgenia Kozorovitskiy

Proceedings Volume PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII; PC123900B (2023) https://doi.org/10.1117/12.2654989
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Oblique plane light-sheet microscopy provides versatile and rapid volumetric functional and structural fluorescence imaging for biology. A single microscope objective illuminates a tilted plane in the specimen and collects emission. Descanned emission is relayed and tilt-corrected through two additional microscope arms to a camera. Due to relative system complexity, estimating required hardware for diverse applications and system alignment can be challenging. Towards overcoming this obstacle and maximizing access, we present an open access scanned oblique plane microscopy platform, along with a design GUI, detailed alignment protocols, control software, application examples in zebrafish and mice, as well as possible systems extensions.

Conference Presentation

Citation Download Citation

Manish Kumar, Jose Ernesto Canton-Josh, and Yevgenia Kozorovitskiy "Open access single objective light-sheet microscopy (Conference Presentation)", Proc. SPIE PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII, PC123900B (16 March 2023); https://doi.org/10.1117/12.2654989

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