Quantitative determination of red blood cell surface and volume is desired for cell classification and experimental verification of theoretical models of the membrane properties of normal human erythrocytes. A direct technique has been developed for measuring profiles at appropriate places on the cell. The method makes use of the electron beam of a scanning electron microscope to mark the cell surface with reference lines which can then be viewed at arbitrary angles to provide unequivocal measurements of the height of the sample. The method also provides a reference to test the linear system properties of optical microscopes.
William A. Simon,
"Direct Measurement Of Red Blood Cell Profiles", Proc. SPIE 0120, Three-Dimensional Imaging, (29 December 1977); doi: 10.1117/12.955751; https://doi.org/10.1117/12.955751