Patients with osteomalacia often complain of back pain or diffuse peripheral bone aches where a mechanical component warrants closer investigations. In order to study the stress-strain patterns in whole human bones and the influence of the degree of calcification upon these patterns, normal bones are submitted to stress and holographic studies are performed prior to and after demineralization. Interpretation of fringes is delicate because the type of loading must be the same in all the experiments with the same bone. Or the demineralization can change non uniformaly the nature of the osseous inhomogeneous structure and caution must be taken to avoid resulting change namely in momentum. Displacements components must be determined in each face of the bone and the calcul of stresses is made in a first step with assumption of equivalent homogeneous material. The problems arrizing in the machanical behaviour of bones are studied experimentally with the various stress analysis techniques. Among these holographical interferometry proved to be particularly well suited because of its high sensitivity, the rough bone surface needs no preparation and it yields the whole deformation field of the surface. Unfortunately the mechanical comportment of the osseous material depends of many factors and the demineralization namely can change non uniformaly the nature of the inhomogeneous structures like bones and caution must be taken for the interpretation of holographic fringes : because of structural variations in bones, apparently similar types of loading can be different namely by evolution of the position of the neutral axis for momentum. Those phenomena will be analysed in different examples. If estimation of demineralization effect is desired, for instance by measurement of appropriated change in elastic modulus, it is important to know any variation in the loading conditions due to specific changes of the material. The first problem considered concerned the measure of Young's modulus E and Poisson's ration of a tigh-bone or tibia. Small test specimens of constant rectangular section are cut out and submitted to pure bending. Classical double exposure holograms are recorded, with the directions of lighting n1 and observation n2 of the specimen nearly parallel to the normal Oz by use of a mirror. (cfr Figure 1).