1 November 1982 Video Imaging System For Microscopy: Technical Aspects
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Abstract
In order to quantitatively study cellular and nuclear properties, we have assembled a low cost video/microscope densitometric system. The system consists of a conventional light microscope coupled to a low light level TV camera whose output is sequentially processed by two microcomputers, one for image acquisition and one for data analysis. The system allows us to a) acquire and store microscope images, b) make spatial measurements such as size and shape, and c) make grayness measurements needed to compute optical density and texture descriptors. Despite the inherent limitations of a video micrometry system (e.g. poor signal-to-noise ratio, automatic gain compensations, etc.) we have been able to collect reproducible data with satisfactory accuracies.
© (1982) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
H. Callisen, H. Callisen, M. Pincu, M. Pincu, A. Norman, A. Norman, } "Video Imaging System For Microscopy: Technical Aspects", Proc. SPIE 0372, Physics and Engineering in Medical Imaging, (1 November 1982); doi: 10.1117/12.934492; https://doi.org/10.1117/12.934492
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