Comparison Of Image Averaging Methods For Structure Determination In Molecular Biology

Olaf Kubler; Dietmar Pum

doi:10.1117/12.936635

9 January 1984 Comparison Of Image Averaging Methods For Structure Determination In Molecular Biology

Olaf Kubler, Dietmar Pum

Proceedings Volume 0432, Applications of Digital Image Processing VI; (1984) https://doi.org/10.1117/12.936635
Event: 27th Annual Technical Symposium, 1983, San Diego, United States

Abstract

Structure determination in molecular biology with the aid of the electron microscope is made difficult by the extreme susceptibility of biological objects to ionizing radiation. Molecular structures may be assessed after averaging over many low dose images of like preparations. Signal averaging is facilitated when periodic structures such as crystalline membrane components are investigated. Fourier techniques akin to methods of X-ray crystallography or matched filter techniques may be used to obtain the average structure. The alternative methods, their reliability and their usage were investigated on computer simulations of electron microscopical images.

Citation Download Citation

Olaf Kubler and Dietmar Pum "Comparison Of Image Averaging Methods For Structure Determination In Molecular Biology", Proc. SPIE 0432, Applications of Digital Image Processing VI, (9 January 1984); https://doi.org/10.1117/12.936635

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