4 December 1984 Reconstruction Of 3D Light Microscopic Images
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Abstract
Light-microscopic images of three-dimensional objects are distorted by defocused projections into the focus plane. A reconstruction procedure for three-dimensional light-microscopic images based on the linear system theory is described. Reconstruction is executed by inverse filtering of the image using an approximation of the three-dimen-sional optical transfer function of the microscope. The derivation and the features of this transfer function are out-lined. The reconstruction procedure permits to achieve a significant improvement of the image quality. The recon-struction result of a test object is shown.
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Gerhard Zinser, Gerhard Zinser, Angelika Erhardt, Angelika Erhardt, Josef Bille, Josef Bille, } "Reconstruction Of 3D Light Microscopic Images", Proc. SPIE 0504, Applications of Digital Image Processing VII, (4 December 1984); doi: 10.1117/12.944882; https://doi.org/10.1117/12.944882
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