20 December 1985 Medicinals Bonded To Biopolymers: Quantitative And Qualitative FTIR Determinations
Author Affiliations +
Proceedings Volume 0553, Fourier and Computerized Infrared Spectroscopy; (1985) https://doi.org/10.1117/12.970761
Event: 1985 International Conference on Fourier and Computerized Infrared Spectroscopy, 1985, Ottawa, Canada
Abstract
We report the successful quantitative determination of small molecules (haptens) to biomacromolecules. Results are shown for two such systems: 7-[4-chloro-3,5-dinitro-benzamido)-deacetoxycephalosporanic acid (CDNB-7-ADCA), coupled to bovine serum albumin, and polyethyleneglycol coupled to ovalbumin. In each case, the number of haptens actually bonded is considerably less than theoretically possible. We also report the successful analysis of three additional systems, but examples of their data are not shown: CDNB-7-ADCA,CDNB, and 3-[CDNB]-2-azetidinone coupled to ovalbumin. In all cases, the fact that pertinent moieties of the hapten are intact indicates that the entire hapten is intact also.
© (1985) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
L. G. Tensmeyer, H. L . Hunter, R. Nagarajan, B. Chao, J . B. Deeter, G. A . Koppel, S. L. Briggs, "Medicinals Bonded To Biopolymers: Quantitative And Qualitative FTIR Determinations", Proc. SPIE 0553, Fourier and Computerized Infrared Spectroscopy, (20 December 1985); doi: 10.1117/12.970761; https://doi.org/10.1117/12.970761
PROCEEDINGS
2 PAGES


SHARE
Back to Top