The Phase Transition Behaviour Of Multibilayer Preparations Of Free Lipid A, The Endotoxic Principle Of The Outer Membrane Of Gram-Negative Bacteria: A FT-IR Study

D. Naumann; Ch. Schultz; H. Labischinski; H. Bradaczek

doi:10.1117/12.970752

20 December 1985 The Phase Transition Behaviour Of Multibilayer Preparations Of Free Lipid A, The Endotoxic Principle Of The Outer Membrane Of Gram-Negative Bacteria: A FT-IR Study

D. Naumann, Ch. Schultz, H. Labischinski, H. Bradaczek

Author Affiliations +

Proceedings Volume 0553, Fourier and Computerized Infrared Spectroscopy; (1985) https://doi.org/10.1117/12.970752
Event: 1985 International Conference on Fourier and Computerized Infrared Spectroscopy, 1985, Ottawa, Canada

Abstract

FT-IR spectra of free lipid A, isolated from Escherichia coli containing C-14 and C-12 acyl chains have been recorded and tentative band assignments were undertaken. The highly complex phase transition behaviour of fully hydrated multibilayer preparations of lipid A has been investigated using temperature-induced frequency shifts, difference spectra and second derivatives of >CH2 asymmetric and symmetric stretching modes near 2918 and 2850 cm -1, respectively. Three "phase transitions" near 22, 34 and 41oC could be detected, the latter being the main transition and - most probably - the familiar B-u-transition. The high value of 41°C found for the main transition temperature may reflect the stability and permeation barrier function of this molecule within the outer membrane of gram-negative bacteria.

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D. Naumann, Ch. Schultz, H. Labischinski, and H. Bradaczek "The Phase Transition Behaviour Of Multibilayer Preparations Of Free Lipid A, The Endotoxic Principle Of The Outer Membrane Of Gram-Negative Bacteria: A FT-IR Study", Proc. SPIE 0553, Fourier and Computerized Infrared Spectroscopy, (20 December 1985); https://doi.org/10.1117/12.970752

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