Optical dosimetry has been investigated in a tissue phantom, consisting of an aqueous suspension of uniform polystyrene spheres plus methylene blue as a chromophore. The rate of light absorption by the dye was measured for different concentrations of dye and scatterers, using the photosensitized inactivation of an enzyme as a sensitive internal actinometer. The optical penetration depth of each sample was measured with a fiber optic probe technique. The results are in good agreement with the predictions of the diffusion model, and the high scattering limit of Kubelka-Munk theory.
Leonard I. Grossweiner,
James W. Messina,
"Model System Studies On Diffuse Absorption", Proc. SPIE 0605, Optical and Laser Technology in Medicine, (5 August 1986); doi: 10.1117/12.956352; https://doi.org/10.1117/12.956352