A simple method of determining the errors in a multicomponent analysis by phase resolved luminescence spectroscopy (PRS) is presented. Without resorting to many tedious experiments, one can determine a priori the optimum phase angles and frequencies for an analysis based only on the component's lifetimes. The approach allows estimation of the theoretical limiting precision of a data acquisition-reduction method. This, in turn, allows assessment of whether different noise sources or systematic errors are present and where improvements in experimental design can enhance the measurement. Finally, it allows direct analysis of the effect of different types of noise sources. An evaluation of the errors in a two-component analysis in the presence of photon noise is presented.
J. N. Demas, J. N. Demas,
"Error Analysis Of Multicomponent Phase-Resolved Luminescence Spectroscopy", Proc. SPIE 0743, Fluorescence Detection, (1 January 1987); doi: 10.1117/12.966919; https://doi.org/10.1117/12.966919