A Real-Time Confocal Laser Scanning Microscope (CLSM)

A. Draaijer; P. M. Houpt

doi:10.1117/12.941501

3 August 1987 A Real-Time Confocal Laser Scanning Microscope (CLSM)

A. Draaijer, P. M. Houpt

Proceedings Volume 0809, Scanning Imaging Technology; (1987) https://doi.org/10.1117/12.941501
Event: Fourth International Symposium on Optical and Optoelectronic Applied Sciences and Engineering, 1987, The Hague, Netherlands

Abstract

A serious disadvantage of most confocal optical scanning microscopes is that they use mechanical scanning methods, which lead to long frame times. Here we describe an off-axis CLSM in which scanning of the laser focal point is achieved with the aid of a fast acousto-optical deflector for line scanning, and a scanning mirror for frame scanning. This arrangement affords short frame-times of 0.05 seconds, and 0.02 seconds is within easy reach. A more or less three-dimensional image of the object can be built up at high speed by combining a number of confocal image sections. The system is suitable for reflection as well as fluorescence microscopy.

Citation Download Citation

A. Draaijer and P. M. Houpt "A Real-Time Confocal Laser Scanning Microscope (CLSM)", Proc. SPIE 0809, Scanning Imaging Technology, (3 August 1987); https://doi.org/10.1117/12.941501

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