Photoaffinity Labeling As A Probe Of Ribosomal Structure And Function

Barry S. Cooperman

doi:10.1117/12.942687

19 February 1988 Photoaffinity Labeling As A Probe Of Ribosomal Structure And Function

Barry S. Cooperman

Proceedings Volume 0847, New Directions in Photodynamic Therapy; (1988) https://doi.org/10.1117/12.942687
Event: Cambridge Symposium on Optics in Medicine and Visual Image Processing, 1987, San Diego, CA, United States

Abstract

Results are presented showing how photoaffinity labeling can be used to identify ribosomal components at functional sites within the Escherichia coli ribosome. Following brief overviews of ribosomal structure and function and of the use of photoaffinity labeling to study complex biological macrostructures, results obtained in the authors' laboratory, principally with the ribosomal antibiotic puromycin and with an aryl azide derivative of puromycin, are presented to illustrate procedures used to identify photoaffinity-labeled ribosomal components, to determine whether such labeling occurs at a functionally important site, and to localize sites of labeling in a three-dimensional sense.

Citation Download Citation

Barry S. Cooperman "Photoaffinity Labeling As A Probe Of Ribosomal Structure And Function", Proc. SPIE 0847, New Directions in Photodynamic Therapy, (19 February 1988); https://doi.org/10.1117/12.942687

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