Optical Fiber System For The Optical Monitoring Of Membrane Potentials Of Excitable Tissues.

G. Nassif; F. Fillette M.D.; R. T. Kado

doi:10.1117/12.945271

21 June 1988 Optical Fiber System For The Optical Monitoring Of Membrane Potentials Of Excitable Tissues.

G. Nassif, F. Fillette M.D., R. T. Kado

Proceedings Volume 0906, Optical Fibers in Medicine III; (1988) https://doi.org/10.1117/12.945271
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

Spectrometry of potential-sensitive dyes permits now the monitoring of membrane potential (MP) variations on excitable tissues. It is also possible to monitor qualitatively the electromechanical activity (EMA) on contractile tissues using this same method. We improved two new optical fiber systems for measuring fluorescence. System 1 used two 200 um optical fibers permitting fluorescence excitation of the potential sensitive dye (PSD) (1st Fiber) and the measurement of its fluorescence (2nd Fiber). System 2 used two 200 um optical fibers for a differencial measurement of excitation reflexion and fluorescence through two different optical filters. The two fibers are assembled in a tube to constitute the "Optrode". In System 1, a single photodiode placed behind a 665 nm high pass optical filter (HPOF) is preamplified by a LH-0022 op. amplifier. In system 2, two photodiodes are placed respectively behind a 645 nm and a 665 nm HPOFs and differencialy preampli-fied by a AD-521 instrumentation amplifier. Analogic signals are lowpass filtered with a LT-1062 digital filter. PSDs WW781 and RH 237 were used respectively on myocardial and nervous tissues. Fluorescence excitation of PSD WW 781 was performed with a 5 mWatts Helium-Neon LASER (He-Ne) beam focused into the first opti-cal fiber of System 1 or illuminating directly the stained tissue. Fluorescence excitation of PSD RH 237 was performed with a 100 Watts filament lamp through a 400-500 nm blue bandpass filter (BBPF). These two systems are now used to study MP variations on myocardial and nervous tissues. We are now able to study the effects of different drugs on the EMA of heart muscle. This technique is usable "in Vitro" and "in Vivo". This optical fiber method easy to improve for a low cost permits now to perform studies on excitable tissues in a non traumatic way with foreseeable applications to pharmacological investigations on experimental models.

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G. Nassif, F. Fillette M.D., and R. T. Kado "Optical Fiber System For The Optical Monitoring Of Membrane Potentials Of Excitable Tissues.", Proc. SPIE 0906, Optical Fibers in Medicine III, (21 June 1988); https://doi.org/10.1117/12.945271

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KEYWORDS

Tissues

Optical fibers

Luminescence

Optical filters

Action potentials

Heart

Tissue optics

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